Select the appropriate test tube according to the item being examined. Blood injection sequence is culture flask, ordinary test tube, test tube containing solid anticoagulant, test tube containing liquid anticoagulant. The purpose of this sequence is to minimize the analytical error caused by the collection of specimens. Blood distribution order: ① Using glass tube order: blood culture tube, no anticoagulant serum tube, sodium citrate anticoagulant tube, other anticoagulant tube. (2) The order of using plastic tubes: blood culture tube (yellow), sodium citrate anticoagulant tube (blue), serum tube with or without adding blood coagulation activator or gel separation, heparin tube with or without adding gel (green), EDTA anticoagulant tube (purple), and blood glucose inhibitor tube (gray). 2. The blood collection site and posture for infants can be recommended by the World Health Organization

The principle of vacuum blood collection is to pre-draw different vacuum degrees of the tube with the head cover, and use its negative pressure to automatically and quantitatively collect venous blood. One end of the blood collection needle is pierced into the human vein and the other end is inserted into the rubber plug of vacuum blood collection. Under the action of negative pressure, the human venous blood is drawn into the blood container through the needle in the vacuum collecting vessel. Under a vein puncture, multi-tube collection can be achieved without leakage. The volume of the inner cavity connected to the blood collection needle is small, so the influence on the blood collection volume can be ignored, but the probability of reflux is relatively small. If the volume of the inner cavity is large, it consumes part of the vacuum of the blood collection vessel, thus reducing the collection amount. [1]1. The red cranial catheter of the common serum tube contains no additives, no

1. The blood sampling needle slips out of the blood vessel: Generally, the blood sampling needle is not fixed or not fixed firmly, which is caused by pulling. Treatment measures: After successful puncture, the needle handle was fixed with tape to prevent the needle handle from sliding, resulting in the needle prolapse. 2. Hemolysis: the vacuum negative pressure is large, and the blood quickly flows into the test tube during the operation, and the hemolysis is caused by the collision between the blood and the wall of the test tube; When the vacuum negative pressure tube with anticoagulant is collected, it swings too violently during the blood collection process, resulting in hemolysis. Treatment measures: the vacuum tube should be tilted 45°, and the inclined plane of the blood sampling needle should be close to the wall of the test tube, so that the blood flows slowly. 3. The vacuum negative pressure tube does not suck blood smoothly or does not suck blood: the main reason is that the

1. Read the instructions carefully before using the vacuum blood collection device, and operate strictly according to the instructions 2. Try to select a thick vein for puncture. 3. After blood collection, first pull out the blood test tube at the end of the puncture plug, and then pull out the puncture needle. 4. Do not loosen the plug of the disposable vacuum blood collection test tube before use, in case the blood collection amount is not allowed 5. If several samples are required at a time, blood should be collected in the following order: blood culture tube, tube without anticoagulant and additive, tube with blood coagulation and tube with anticoagulant. 6. The latex sleeve of the needle end of the plug can prevent the blood from continuing to pollute the surrounding after pulling out the blood test tube, so as to prevent the pollution of the environment by closing the blood collection, so it is not advisable to drop the latex sleeve.